Abstract

Durum wheat highly depends on nitrogen for seed development and yield, and the obtainmentof varieties with a better nitrogen use efficiency is crucial to reduce production costsand environmental pollution. In this study, sequencing of two small RNA libraries obtainedfrom tissues of Ciccio and Svevo cultivars grown under nitrogen starvation conditions produced84 novel, and 161 conserved miRNAs. Of these, 7 novel and 13 known miRNAs werenewly identified in this work. Quantitative PCR analysis of selected miRNAs highlighted thatthe expression levels of some of them depends on the tissue and on the cultivar, Svevobeing the most responsive to nitrogen starvation. A number of target genes were predictedto be involved in nitrogen metabolism. An inverse correlation for the qPCR expression dataof miRNA/target pairs miR399b/PHO2, miR393c/AFB2, ttu-novel-61/CCAAT-TF wasobserved in specific tissues or cultivar. Especially, ttu-novel-61 was down-regulated and itstarget CCAAT-TF up-regulated in almost all tissues both in Svevo and in Ciccio. Moreover,CCAAT-TF was confirmed to be cleaved by ttu-novel-61 at the expected site. The discoveryof miRNAs involved in the response to nitrogen stress represents an important step towardsfunctional analyses, with the final aim to design strategies for improving nitrogen use efficiencyin durum wheat.

Autore Pugliese

• Sonnante Gabriella

Tutti gli autori

• Zuluaga D.L.; De Paola D.; Janni M.; Curci P.L.; Sonnante G.

Titolo volume/Rivista

PloS one

Anno di pubblicazione

2017

ISSN

1932-6203

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile