Dispersive Raman spectroscopy at 1064 nm for rapid screening of deoxynivalenol in wheat bran: preliminary results.

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Abstract

Deoxynivalenol (DON) is a Fusarium toxin which frequently occurs in grains. Because of the toxic effectsinduced by DON, many regulations worldwide have established safety levels in food and feed. For instance,the EC maximum limit for DON in unprocessed wheat bran has been set at 750 ?g/kg. New devices areenvisaged for the rapid detection of DON in grain stocks in order to verify the compliance with EUregulation and to perform a quick assessment of contamination without using chemicals and benchanalytical instruments. Optical spectroscopy is currently emerging as a modern and "green" analyticaltechnique for intact food analyses, thanks to the non-destructive nature of light measurements whichenable rapid checks without making use of reagents or chemical treatments, thus avoiding the problem ofwaste disposal.The objective of this study was to assess the use of Raman spectroscopy, excited at 1064 nm by using adispersive detection scheme, for rapid screening of DON in wheat bran. Twelve wheat bran samplescontaminated with DON in the range <=100-1600 ?g/kg were considered. Four replica measurements werecarried out for each sample, thus taking into account unavoidable inhomogeneity of contamination. Ramanspectra were processed using Standard Normal Variate (SNV) and Orthogonal Signal Correction (OSC) forcompensation of scattering influence, and removal of DON-independent effects. Then, Partial Least Squareregression was applied as a predictive model for DON quantification. A coefficient of determinationR2=0.72 was obtained, together with a root means square error of calibration RMSEC=313 ?g/kg, thusindicating that Raman spectroscopy has good potential as a rapid tool for DON detection.

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  • Ciaccheri L.; Mignani A.G.; Mencaglia A.A.; De Girolamo A.; Lippolis V.; Pascale M.

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2014

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