Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films

Abstract

Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14?g/cm<sup>2</sup> as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.


Tutti gli autori

  • Palumbo F.; Camporeale G.; Yang Y.-W.; Wu J.-S.; Sardella E.; Dilecce G.; Calvano C.D.; Quintieri L.; Caputo L.; Baruzzi F.; Favia P.

Titolo volume/Rivista

Plasma processes and polymers


Anno di pubblicazione

2015

ISSN

1612-8869

ISBN

Non Disponibile


Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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