Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films
Abstract
Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14?g/cm<sup>2</sup> as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.
Autore Pugliese
Tutti gli autori
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Palumbo F.; Camporeale G.; Yang Y.-W.; Wu J.-S.; Sardella E.; Dilecce G.; Calvano C.D.; Quintieri L.; Caputo L.; Baruzzi F.; Favia P.
Titolo volume/Rivista
Plasma processes and polymers
Anno di pubblicazione
2015
ISSN
1612-8869
ISBN
Non Disponibile
Numero di citazioni Wos
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Numero di citazioni Scopus
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Settori ERC
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Codici ASJC
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