Abstract

Citrus, one of the most economicallyimportant crops, is susceptible to a number ofarthropod- and graft-transmissible pathogens. Rapidand reliable methods for detecting multiple pathogensare important for routine diagnosis by reducing time,labour and costs. To this end, primers and TaqManprobes for Citrus psorosis virus (CPsV) and Citrusvariegation virus (CVV) detection by singleplex realtime(q) reverse transcription (RT)- PCR were initiallydesigned. Further optimizations included the developmentof a multiplex (m) RT-qPCR assay to detectsimultaneously CPsV, CVV, and Citrus tristeza virus(CTV) in a single reaction. When 10-fold serialdilutions prepared using total RNAs from CPsV- andCVV-infected plants were tested, RT-qPCR assaysproved to be 100 and 1000 times more sensitive thanconventional RT-PCR, respectively. The target viruseswere effectively identified by mRT-qPCR in fieldinfectedclementine and sweet orange trees. Theoptimized multiplex assay proved to be as sensitiveas the singleplex tests, thus providing a valuablealternative tool for detection of these citrus viruses.

Autore Pugliese

• Saponari Maria

Tutti gli autori

• G. Loconsole; M. Saponari; V. Savino

Titolo volume/Rivista

European journal of plant pathology

Anno di pubblicazione

2010

ISSN

0929-1873

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile