Abstract

A Mass Spectrometry ImmunoAssay (MSIA) specifically designed for the detection of egg allergens in wines is described. This approach is based on an immunoaffinity enrichment procedure combined with targeted MS/MS detection of selected egg peptide markers. Polyclonal antibodies raised against native ovalbumin, chosen as the target protein tracing for egg powder, were immobilized onto low backpressure monolithic MSIA customized disposable tips. Ovalbumin-free wine samples were fortified with standard protein at different concentration levels in the low µg/mL range. A simple protocol was devised consisting in a 1:4 dilution of the wine sample with a basic solution for pH adjustment, followed by a semi-automated purification/enrichment step on customized MSIA disposable tips fitted on a multichannel electronic pipette. Among the main figures of merit LOD and LOQ values as low as 0.01 and 0.03 ?g/mL, respectively, of 18 % should be noticed. Noteworthy, the developed assay outperformed current MS-based methods for the detection of allergenic protein in wine matrices, thanks to the immunoaffinity enrichment. In addition compared to other immunoassays, the present approach boasts the unquestionable advantage to provide an unambiguous identification of the target protein, by simultaneous detection of three unique peptide markers each giving three specific MS/MS transitions.

Autore Pugliese

• Monaci Linda , Pilolli Rosa

Tutti gli autori

• R. PILOLLI; R. CHAUDHARI; F. PALMISANO; L. MONACI

Titolo volume/Rivista

Analytical & bioanalytical chemistry

Anno di pubblicazione

2016

ISSN

1618-2642

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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