Abstract

A new analytical method for the determination of ochratoxin A (OTA) in red wine has been developed by using adouble-extract cleanup and a fluorometric measurement after spectral deconvolution. Wine samples were diluted with a solutioncontaining 1% polyethylene glycol and 5% sodium hydrogencarbonate, filtered, and purified by immunoaffinity and aminopropylsolid-phase column. OTA contents in the purified extract were determined by a spectrofluorometer (excitation wavelength, 330nm; emission wavelength, 470 nm) after deconvolution of fluorescence spectra. Average recoveries from wine samples spikedwith OTA at levels ranging from 0.5 to 3.0 ng/mL were 94.5-105.4% with relative standard deviations (RSD) of <15% (n = 4).The limit of detection (LOD) was 0.2 ng/mL, and the total time of analysis was 30 min. The developed method was tested on 18red wine samples (naturally contaminated and spiked with OTA at levels ranging from 0.4 to 3.0 ng/mL) and compared withAOAC Official Method 2001.01, based on immunoaffinity column cleanup and HPLC with fluorescence detector. A goodcorrelation (r2 = 0.9765) was observed between OTA levels obtained with the two methods, highlighting the reliability of theproposed method, the main advantage of which is the simple OTA determination by a benchtop fluorometer with evidentreductions of cost and time of analysis.

Autore Pugliese

• Panzarini Giuseppe , Pascale Michelangelo

Tutti gli autori

• Longobardi F.; Iacovelli V.; Catucci L.; Panzarini G.; Pascale M.; Visconti A.; Agostiano A.

Titolo volume/Rivista

Journal of agricultural and food chemistry

Anno di pubblicazione

2013

ISSN

0021-8561

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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