Abstract

A DNA aptamer with high affinity and specificity to ochratoxin A (OTA) was conjugated to a coupling geland used as sorbent for the preparation of solid phase extraction (SPE) columns. The SPE columns packedwith 300 ll oligosorbent (24 nmol DNA) showed a linear (r = 0.999) behaviour in the range of 0.4-500 ngOTA. After optimisation of the extraction step, SPE columns were used for clean-up of OTA from wheatprior to liquid chromatographic (HPLC) analysis with fluorescence detection (FLD). Average recoveriesfrom wheat samples spiked at levels of 0.5-50 ng/g ranged from 74% to 88% (relative standard deviation<6%) with limits of detection and of quantification of 23 and 77 pg/g, respectively. The comparativeHPLC/FLD analyses of 33 naturally contaminated durum wheat samples cleaned-up on both aptamer-SPE and immunoaffinity (IMA) columns showed a good correlation (r = 0.990). Aptamer-SPE columnscould be re-used up to five times without any loss of performance.

Autore Pugliese

• De Girolamo Annalisa

Tutti gli autori

• De Girolamo A.; McKeague M.; Miller J.D.; De Rosa M.C.; Visconti A.

Titolo volume/Rivista

Food chemistry

Anno di pubblicazione

2011

ISSN

0308-8146

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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