Abstract

A liquid chromatographic method for thedetermination of fumonisins B1 (FB1) and B2(FB2) in corn-based foods for infants and youngchildren was subjected to an interlaboratoryvalidation study involving 11 laboratories. Fiveblind duplicate sample pairs of each matrixwere analyzed to establish the accuracy,repeatability, and reproducibility of the method.Mass fractions in the baby food samplesranged from 89.1 to 384.4 ?g/kg FB1 and from22.5 to 73.6 ?g/kg FB2. The method involved awarm extraction with citrate phosphate buffer-methanol-acetonitrile (50 + 25 + 25, v/v/v), acleanup through an immunoaffinity column,and an end-determination of fumonisins by LCafter automated precolumn derivatization witho-phthaldialdehyde reagent. RSDs for withinlaboratoryrepeatability (RSDr) ranged from 6.8to 23.5% for FB1 and 7.6 to 22.9% for FB2. RSDsfor between-laboratory reproducibility (RSDR)ranged from 15.4 to 26.2% for FB1 and 21.6 to36.3% for FB2. Mean FB1 recoveries from babyfoods spiked at 100.0 and 250.0 ?g/kg were 89and 96%, respectively; for FB2 spiked foods at25.0 and 62.5 ?g/kg recoveries were 90 and 85%,respectively. HorRat values ranged from 0.8 to1.2 for FB1, whereas for FB2 they ranged from0.9 to 1.4 when calculated according to Horwitz,and from 1.0 to 1.7 when calculated accordingto Thompson, indicating an acceptable amonglaboratoryprecision for all matrixes (HorRatvalues <2).

Autore Pugliese

• De Girolamo Annalisa , Gambacorta Lucia , Solfrizzo Michele

Tutti gli autori

• Solfrizzo M.; De Girolamo A.; Gambacorta L.; Visconti A.; van Egmond H.P.; Stroka J.

Titolo volume/Rivista

Journal of AOAC International

Anno di pubblicazione

2011

ISSN

1060-3271

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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