Determination of deoxynivalenol, T-2 and HT-2 toxins in a bread model food by liquid chromatography - high resolution - Orbitrap - Mass Spectrometry equipped with a high-energy collision dissociation cell.

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Abstract

A sensitive and accurate method employing a single stage high resolution mass spectrometer equippedwith a high-energy collision-dissociation cell (HCD) for the simultaneous determination of deoxynivalenol(DON), T-2 toxin (T-2) and HT-2 toxin (HT-2) in a processed bread model food has been developed.Two sample pre-treatment routes for the extraction of these mycotoxins were investigated, based onMycosep® column clean up or QuEChERS-like procedure, respectively. The former approach suffered lessfrom matrix effects and allowed to achieve in bread samples LODs of 7, 12 and 17 ng/g for T-2, HT-2and DON, respectively, with 0.5 ppm mass accuracy. Two acquisition modes, full scan MS and all ionfragmentation, exploiting the fragmentation features offered by an HCD chamber and integrated withinthe Orbitrap analyser, were compared for quantitative purposes. The method was applied to investigatethe degradation of these mycotoxins during bread processing using a bread model food. Most T-2hydrolyzed to HT-2 during dough preparation, and about 20-30% of HT-2 and DON was degraded duringbread baking.

Autore Pugliese

Tutti gli autori

  • Monaci L.; De Angelis E.; Visconti A.

Titolo volume/Rivista

Journal of chromatography

Anno di pubblicazione

2011

ISSN

0021-9673

ISBN

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Numero di citazioni Wos

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Settori ERC

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