Abstract

Available data on the incidence and biodiversity of pome fruit viroids in the Mediterranean basin are limited. Beforestarting a research survey to fill this gap, a tissue-printing hydridization (TPH) method to detect Apple scar skin viroid(ASSVd), Pear blister canker viroid (PBCVd) and Apple dimple fruit viroid (ADFVd) has been developed andvalidated. Afterward, TPH was used in large-scale indexing of pome fruit viroids in Bosnia and Herzegovina, Malta,Lebanon and Turkey. A total of about 1,000 trees was randomly collected and tested. Positive results obtained by TPHwere confirmed by at least one additional detection method (RT-PCR and/or Northern-blot hybridization) and viroidswere finally identified by sequencing full-length cDNA clones. PBCVd was detected in 13%, 12.4% and 5.4% of thetested pear trees in Bosnia and Herzegovina, Malta and Turkey, respectively, showing a wider diffusion of this viroidthan expected. In contrast, ASSVd was never detected and ADFVd was only found in symptomatic trees (cv. StarkingDelicious) in Lebanon, confirming a restricted presence of these viroids in the Mediterranean basin. Altogether, thesedata support the use of TPH as an easy and valuable tool for exploring pome fruit viroid spread.

Autore Pugliese

• Navarro Ramirez Beatriz , Di Serio Francesco

Tutti gli autori

• DI SERIO F.; AFECHTAL M.; ATTARD D.; CHOUEIRI E.; GUMUS M.; KAYMAK S.; LOLIC B.; MATIC S.; NAVARRO B.; YESILCOLLOU S.; MYRTA A.

Titolo volume/Rivista

Julius Khun Archiv

Anno di pubblicazione

2010

ISSN

1868-9892

ISBN

978-3-930037-67-4

Numero di citazioni Wos

Nessuna citazione

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