Detection and molecular characterization of a Grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant in an autochthonous grape from Apulia (Italy)

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Abstract

The complete nucleotide sequence and genome organization of a new Badnavirus isolated from the autochthonous grapevine variety ''Bombino nero'' from Apulia (Italy) was determined. The genome of this virus consists of 7097 nt and has four open reading frames (ORFs). Analysis of putative proteins encoded by each ORF revealed greatest sequence similarity to Grapevine Roditis leaf discolorationassociated virus w4 (GRLDaV; NC_027131). In a pairwise alignment with GLRDaV w4 genome sequence, the ''Bombino Nero'' sequence was 109 nt longer with a major 57 nt insertion between positions 2405 and 2413. Furthermore, its putative ORF4 is located after the ORF3, while in the GLRDaV w4 sequence, the putative ORF4 completely overlapped ORF3. Nucleotide analysis classifies this new Badnavirus as a GLRDaV strain, which was named GRLDaV-BN. Multi-year field observations showed that the GLRDaV-BN-infected vine was symptomless.

Autore Pugliese

Tutti gli autori

  • M. Chiumenti; A. Giampetruzzi; M. Morelli; V. N. Savino; G. P. Martelli; P. La Notte; F. Palmisano; P. Saldarelli

Titolo volume/Rivista

Virus genes

Anno di pubblicazione

2016

ISSN

1572-994X

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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