Abstract

Virus-derived short interfering RNAs (vsiRNAs) isolated from grapevine V. vinifera Pinot Noir clone ENTAV115 were analyzed by high-throughput sequencing using the Illumina Solexa platform. We identified andcharacterized vsiRNAs derived from grapevine field plants naturally infected with different viruses belongingto the genera Foveavirus, Maculavirus, Marafivirus and Nepovirus. These vsiRNAs were mainly of 21 and 22nucleotides (nt) in size and were discontinuously distributed throughout Grapevine rupestris stem-pittingassociated virus (GRSPaV) and Grapevine fleck virus (GFkV) genomic RNAs. Among the studied viruses, GRSPaVand GFkV vsiRNAs had a 52 terminal nucleotide bias, which differed from that described for experimental viralinfections in Arabidopsis thaliana. VsiRNAs were found to originate from both genomic and antigenomicGRSPaV RNA strands, whereas with the grapevine tymoviruses GFkV and Grapevine Red Globe associatedvirus (GRGV), the large majority derived from the antigenomic viral strand, a feature never observed in otherplant–virus interactions.

Autore Pugliese

• Saldarelli Pasquale , Pantaleo Vitantonio

Tutti gli autori

• Pantaleo V.; Saldarelli P.; Miozzi L.; Giampetruzzi A.; Gisel A.; Moxon S.; Dalmay T.; Bisztray G.; Burgyan J.

Titolo volume/Rivista

Virology

Anno di pubblicazione

2010

ISSN

0042-6822

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile