Abstract

An unprecedented, environmentally friendly, and faster method for the determination ofOchratoxin A (OTA) (a mycotoxin produced by several species of Aspergillus and Penicillium andlargely widespread in nature, in wheat and derived products) has, for the first time, been set up andvalidated using choline chloride (ChCl)-based deep eutectic solvents (DESs) (e.g., ChCl/glycerol(1:2) and ChCl/ urea (1:2) up to 40% (w/w) water) as privileged, green, and biodegradable extractionsolvents. This also reduces worker exposure to toxic chemicals. Results are comparable to thoseobtained using conventional, hazardous and volatile organic solvents (VOCs) typical of the standardand official methods. OTA recovery from spiked durum wheat samples, in particular, was to up to89% versus 93% using the traditional acetonitrile-water mixture with a repeatability of the results(RSDr) of 7%. Compatibility of the DES mixture with the antibodies of the immunoaffinity columnwas excellent as it was able to retain up to 96% of the OTA. Recovery and repeatability for durumwheat, bread crumbs, and biscuits proved to be within the specifications required by the currentEuropean Commission (EC) regulation. Good results in terms of accuracy and precision wereachieved with mean recoveries between 70% (durum wheat) and 88% (bread crumbs) and an RSDrbetween 2% (biscuits) and 7% (bread).

Autore Pugliese

• Logrieco Antonio Francesco , Solfrizzo Michele

Tutti gli autori

• L. Piemontese; F.M. Perna; A. Logrieco; V. Capriati; M. Solfrizzo

Titolo volume/Rivista

Molecules

Anno di pubblicazione

2017

ISSN

1420-3049

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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