Abstract

A primary therapeutic goal in Alzheimer’s disease (AD) is to reduce the quantity of amyloid b protein (A b) present in the brain. To develop an effective, safe system for vaccination against Alzheimer’s disease, the plant virus Cucumber mosaic virus (CMV) was engineered genetically to express A b -derived fragments that stimulate mainly humoral immune responses. Six chimeric constructs, bearing the A b 1–15 or the A b 4–15 sequence in positions 248, 392 or 529 of the CMV coat protein (CP) gene, were created. Viral products proved to be able to replicate in their natural host. However, only chimeric A b 1–15-CMVs were detected by A b 1–42 antiserum in Western blot analysis. Experimental evidence of Immunoelectron microscopy revealed a complete decoration of A b 1–15-CMV248 and A b 1–5-CMV392 following incubation with either anti-A b 1–15 or anti-A b 1–42 polyclonal antibodies. These two chimeric CMVs appear to be endowed with features making them possible candidates for vaccination against Alzheimer’s disease.

Autore Pugliese

• De Stradis Angelo , Boscia Donato

Tutti gli autori

• Vitti A.; Piazzolla G.; Condelli V.; Nuzzaci M.;Lanorte M.T.; Boscia D.; De Stradis A.; Antonaci S.; Piazzolla P.; Tortorella C.

Titolo volume/Rivista

Journal of virological methods

Anno di pubblicazione

2010

ISSN

0166-0934

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile