Abstract

The complete nucleotide sequence and the genome organization were determined of a putative newmember of the family Tymoviridae, tentatively named Olive latent virus 3 (OLV-3), recovered in southernItaly from a symptomless olive tree. The sequenced ssRNA genome comprises 7148 nucleotides excludingthe poly(A) tail and contains four open reading frames (ORFs). ORF1 encodes a polyprotein of 221.6 kDain size, containing the conserved signatures of the methyltransferase (MTR), papain-like protease (PRO),helicase (HEL) and RNA-dependent RNA polymerase (RdRp) domains of the replication-associated proteinsof positive-strand RNA viruses. ORF2 overlaps completely ORF1 and encodes a putative protein of43.33 kDa showing limited sequence similarity with the putative movement protein of Maize rayado finovirus (MRFV). ORF3 codes for a protein with predicted molecular mass of 28.46 kDa, identified as thecoat protein (CP), whereas ORF4 overlaps ORF3 and encodes a putative protein of 16 kDa with sequencesimilarity to the p16 and p31 proteins of Citrus sudden death-associated virus (CSDaV) and Grapevinefleck virus (GFkV), respectively. Within the family Tymoviridae, OLV-3 genome has the closest identitylevel (49-52%) with members of the genus Marafivirus, from which, however, it differs because of thediverse genome organization and the presence of a single type of CP subunits.

Autore Pugliese

• Minafra Angelantonio , Saponari Maria

Tutti gli autori

• A. Alabdullah ; A. Minafra; T. Elbeaino ; M. Saponari ; V. Savino;G.P. Martelli

Titolo volume/Rivista

Virus research

Anno di pubblicazione

2010

ISSN

1872-7492

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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