Abstract

Immunofluorescence techniques on formalin fixedparaffin-embedded sections allow for the evaluation of the expressionand spatial distribution of specific markers in patient tissuespecimens or for monitoring the fate of labeled cells after in vivoinjection. This technique suffers however from the auto-fluorescencebackground signal of the embedded tissue that eventuallyconfounds the analysis. Here we show that rod-like semiconductornanocrystals (QRs), intramuscularly injected in living mice, couldbe clearly detected by confocal microscopy in formalin fixedparaffin-embedded tissue sections. Despite the low amount ofQRs amount injected (25 picomoles), these were clearly visibleafter 24 h in the muscle sections and their fluorescence signal wasstronger than that of CdSe/ZnS quantum dots (QDs) similarlyfunctionalized and in the case of QRs only, the signal lasted evenafter 21 days after the injection.

Autore Pugliese

• Quarta Alessandra

Tutti gli autori

• A. Zacheo; A. Quarta; A. Mangoni; P.P. Pompa; R. Mastria;M.C. Capogrossi; R. Rinaldi; T. Pellegrino

Titolo volume/Rivista

IEEE transactions on nanobioscience

Anno di pubblicazione

2011

ISSN

1536-1241

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile