Biological and molecular characterization of a recombinant isolate of Watermelon Mosaic Virus associated with a watermelon necrotic disease in Italy.

Abstract

The biological and molecular characterization is reported of a Watermelon mosaic virus isolate,denoted WMV-Le, associated with a necrotic phenotype of watermelon plants grown in the Provinces ofLecce and Taranto (Apulia, southern Italy). The fully sequencedWMV-Le genome consists of 10,045 nucleotidesand is 99.1% similar to that of WMV-C05-270, a French isolate from melon of the WMV moleculargroup 3. Using recombination detection programRDP3, putative recombination breakpoints were identifiedclose to nucleotide positions 42 to 1892, covering the 5? UTR/P1/HC-Pro region. The event represents theinsertion of a sequence fragment of an isolate similar to WMV-FBR04-37 in the background of an isolate similarto WMV-FMF00-LL1. The field symptomatology was reproduced in watermelon plants grown in an experimental greenhouse but the virus induced severe symptoms also in Cucumis sativus, C. melo, Cucurbita maxima and C. pepo.


Tutti gli autori

  • Finetti-Sialer M.M.; Mascia T.; Cillo F.; Vovlas C.; Gallitelli D.

Titolo volume/Rivista

European journal of plant pathology


Anno di pubblicazione

2012

ISSN

1573-8469

ISBN

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Settori ERC

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Codici ASJC

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