Biodiversity and safety aspects of yeast strains characterized from vineyards and spontaneous fermentations in the Apulia Region, Italy

Abstract

This work is the first large-scale study on vineyard-associated yeast strains from Apulia (Southern Italy).Yeasts were identified by Internal Transcribed Spacer (ITS) ribotyping and bioinformatic analysis. Thepolymorphism of interdelta elements was used to differentiate Saccharomyces cerevisiae strains. Twentydifferent species belonging to 9 genera were identified. Predominant on the grape surface wereMetschnikowia pulcherrima, Hanseniaspora uvarum and Aureobasidium pullulans, whereas M. pulcherrimaand H. uvarum were dominant in the early fermentation stage. A total of 692 S. cerevisiae isolates wereidentified and a number of S. cerevisiae strains, ranging from 26 to 55, was detected in each of the eightfermentations. The strains were tested for biogenic amines (BAs) production, either in synthetic media orgrape must. Two Pichia manshurica, an Issatchenkia terricola and a M. pulcherrima strains were able toproduce histamine and cadaverine, during must fermentation. The production of BAs in wine must wasdifferent than that observed in the synthetic medium. This feature indicate the importance of an "ingrape must" assessment of BAs producing yeast. Overall, our results suggest the importance of microbiologicalcontrol during wine-making to reduce the potential health risk for consumer represented bythese spoilage yeasts.


Tutti gli autori

  • Tristezza M.; Vetrano C.; Bleve G.; Spano G.; Capozzi V.; Logrieco A.; Mita G.; Grieco F.

Titolo volume/Rivista

Food microbiology


Anno di pubblicazione

2013

ISSN

0740-0020

ISBN

Non Disponibile


Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile


Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile


Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile