Abstract

Food authentication and traceability is one of the major concern to the food industry, strictly correlatedto food fraud and food adulteration. Rice (Oryza sativa L.) is one of the most important crops, supplyingfood for over half of the world's population. Authenticity of rice products has become a key issue in thefood industry addressed to protect the interests of quality conscious consumers, stakeholders, andimporting countries. DNA markers offer a powerful tool to address the validation of food authenticityand traceability of primary products. Progress in NGS technology has provided opportunities to detectlarge number of DNA polymorphisms, even in the closely related cultivars. In this study, a whole-genomesequencing of Italian rice cultivar Carnaroli has been carried out from genetically pure certified seed.The sequencing yielded about 22.5 million reads. After quality trimming 21.5 million reads were mappedonto the reference sequence of Oryza sativa ssp. japonica cv. Nipponbare (IRGSP-1.0), providing about90% coverage of the rice genome and an average coverage of 15.12x. Preliminary results, found 450,414candidate DNA polymophisms between cultivar Nipponbare and Carnaroli. These were classified into383,080 SNPs (85%) and 67,334 InDels (15%) by polymorphism types, 150,688 homozygous (85%) and299,726 (15%) heterozygous by zygosity type, 371,801 intergenic (82.5%) and 78613 (17.6%) by genomiclocation. The distribution of DNA polymorphisms was found to be uneven across and within the ricechromosomes. In particular, chromosome 8 and 10 showed the highest density of DNA polymorphisms(14.7% and 14.5%, respectively). This study represents the first report of whole genome sequencing ofItalian rice cultivar Carnaroli and will contribute to develop targeted and un-targeted method for riceauthentication and traceabilityThis work has been supported by the European project FOODINTEGRITY (FP7-KBBE-2013-single-stage, No 613688).

Autore Pugliese

• Mule' Giuseppina , Logrieco Antonio Francesco , Ferrara Massimo , De Paolis Angelo

Tutti gli autori

• M. FERRARA; A. DE PAOLIS; A. F. LOGRIECO; GIUSEPPINA MULÈ

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Anno di pubblicazione

2017

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