Abstract

Wheat is one of the most widely growncereal crops based on the amount of calories itprovides in the human diet. Durum wheat (Triticumturgidum ssp. durum) is largely used for production ofpasta and other products. In order to use geneticknowledge to improve the understanding of N-useefficiency, we carried out, for the first time in durumwheat, the isolation and the characterization of fourmembers of the asparagine synthetase (AsnS) genefamily. Phylogenetic inference clustered the Ttu-AsnS1 (1.1 and 1.2) and Ttu-AsnS2 (2.1 and 2.2)genes in AsnS gene class I, which is present inmonocots and dicots. Class I genes underwent asubsequent duplication leading to the formation of twosubgroups. Plants of Svevo cultivar were grown underN-stress conditions and expression of the four AsnSgenes was investigated at three developmental stages(seedling, booting, and late milk development), crucialfor N absorption, assimilation and remobilization.AsnS1 genes were down-regulated in N-stressed roots,stems and leaves during seedling growth and booting,but seemed to play a role in N remobilization in flagleaves during grain filling. AsnS2 genes were scarcelyexpressed in roots, stems, and leaves. In N-stressedspikes there was no differential expression in any ofthe genes. The genes were mapped in silico using adurum wheat SNP map, assigning Ttu-AsnS1 genes tochromosome 5 and Ttu-AsnS2 to chromosome 3.These findings provide a better understanding of therole of ASN genes in response to N stress in durumwheat.

Autore Pugliese

• Sonnante Gabriella

Tutti gli autori

• P.L. Curci; H. Bergès; W. Marande; M. Maccaferri;R. Tuberosa; G. Sonnante

Titolo volume/Rivista

Euphytica

Anno di pubblicazione

2018

ISSN

0014-2336

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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