Abstract

A very large amount of phenol-polluted waters is formed from the productionof olive oil (olive mill waste water, OMWW) and their disposal represents a seriousproblem. The aim of this study was to investigate the use of peroxidase (POD) fromartichoke (Cynara cardunculus L. subsp. scolymus (L.) Hayek) leaves to removephenols present in OMWW. The POD catalyzes the polymerization andprecipitation of aqueous phenols. This application could be useful, because in theMediterranean region a large amount of OMWW as well as a lot of artichoke scrapsresulting from artichoke processing industry, are produced. So, it is possible toconsider this biomass (leaves and external bud bracts) as a useful and cheap sourceof peroxidase. The enzyme extraction was performed by homogenization andseparation of pigments using an aqueous two phase polymer system (polyethyleneglycol/(NH4)2SO4), that allowed the accumulation of POD in the salt phase. Theoptimal reaction conditions were evaluated. The phenolic concentration was assayedby Folin-Ciocalteu method. Preliminary results showed that the optimal conditionsfor enzyme activity were in a broad range of both pH (from 4 to 7) and temperature(from 5 to 50°C). In optimal enzymatic conditions, a reduction of more than 60% ofinitial phenolic concentration was observed.

Autore Pugliese

• Cardinali Angela , Di Venere Donato , Sergio Lucrezia , Linsalata Vito

Tutti gli autori

• Sergio L.; Di Venere D.; De Paola A.; Linsalata V.; Cardinali A.; Tursi N.; Vanadia S.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2011

ISSN

Non Disponibile

ISBN

978 90 6605 674 9

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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