Artichoke microRNAs involved in the response to biotic stresses

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Abstract

Plant response to environmental stresses and pathogen attacks involves several biologicalprocesses that require fine and precise regulation at transcriptional and post-transcriptional levels.MicroRNAs (miRNAs) are small RNAs (sRNAs) widely diffused in animals and plants andimplicated in post-transcriptional regulation of gene transcripts. Identification of differentiallyexpressed miRNAs, following infection, may provide an insight into the processes involved insignalling and defence of plants against pathogens.In order to characterize artichoke miRNAs differentially expressed during fungus or virusinfection, we sequenced five sRNA libraries obtained from artichoke using Illumina technology.Libraries were obtained from leaves and roots of control plants, and from plants infected withTomato spotted wilt virus (TSWV), or Verticillium dahliae.After removing low quality reads and adapter sequences, all artichoke libraries wereannotated according to small noncoding RNAs contained in Rfam, and all previously known plantmiRNAs extracted from the miRNA Registry Database (miRBase Release 18).Change in miRNA read counts between infected and non infected artichoke tissues wasrecorded and used to select putative infection-responsive miRNAs. Differential expression ofmiRNAs was validated by quantitative real-time PCR (qPCR).Artichoke miRNA precursors were identified and their fold-back structure was predictedusing Mfold program. Artichoke miRNA target genes were also identified and characterizedaccording to the homologous Arabidopsis proteins.In conclusion, miRNAs involved in the response to virus or fungus infection were detectedand validated in artichoke plant tissues.

Autore Pugliese

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  • De Paola D.; Finetti-Sialer M.M.; Catalano D.; Sonnante G.

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Anno di pubblicazione

2012

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Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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