Application of lactoferricin B to control microbial spoilage in cold stored fresh foods
Abstract
Psychrotrophic Pseudomonas spp. were proved to cause dramatic quality losses in fresh foods like leafy greens and dairy products resulting in the shortening of their shelf-life. These spoilage microorganisms are resistant to most disinfection treatments. This work was addressed to assay the antimicrobial efficacy of the food-grade bovine lactoferrin (BLF) and its hydrolysates (LFH), obtained after digestion with pepsin or the enzymatic cardoon extract. The inhibition assays were carried out in vitro on selected Pseudomonas spp. strains involved in off-color development in ready-to-eat vegetables (RTE) and in mozzarella cheese. A higher antimicrobial activity against most of the tested strains was found for pepsin digested BLF in comparison with BLF digested with the enzymatic cardoon extract. A solution of the antimicrobial peptide lactoferricin B (LFcinB), purified from the pepsin-LFH, was applied to both cold stored RTE lettuce leaves and mozzarella cheeses, inoculated with the spoilage strains. A significant reduced tissue browning of RTE lettuce leaves, as well as absence of mozzarella cheese discoloration applying LFcinB. The results obtained show the possibility to use milk-derived antimicrobial peptides in the control of food spoilage caused by Pseudomonas strains.
Autore Pugliese
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Quintieri L.; Baruzzi F.; Caputo L.; Calabrese N.
Titolo volume/Rivista
Non Disponibile
Anno di pubblicazione
2015
ISSN
Non Disponibile
ISBN
978-1-62734-544-6
Numero di citazioni Wos
Nessuna citazione
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Numero di citazioni Scopus
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Settori ERC
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Codici ASJC
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