Abstract

We describe the application of a novel genome walking (GW) strategy for the one-shot identification ofmembers of multigene families. The method was used to study the spinach Lhcb1 family (encoding thelight harvesting complex protein Lhcb1), for which three cDNAs were known. Two additional genes andregulatory regions of the five members of the family were identified. For one of the newly detectedgenes, sequencing of full-length cDNA and analysis of regulatory regions by gel-shift are also reported.To our best knowledge, this is the first report on the use of a GW approach for the study of amultigene family.

Autore Pugliese

• Ceci Luigi Ruggiero

Tutti gli autori

• Leoni C.; Volpicella M.; Placido A.; Gallerani R.; Ceci L.R.

Titolo volume/Rivista

Journal of plant physiology

Anno di pubblicazione

2010

ISSN

0176-1617

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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