Abstract

Echinoderms represent one interesting marine renewable resource and produce bioactive compounds related to their innate immune system.These invertebrates indeed are able to differentiate self from non-self through the production of soluble molecules and coelomocytes response playing animportant role in the resistance to disease.Therefore, they appear as a promising alternative valuable source of new compounds for drug development. In particular, the application of new marine antioxidants in foods, food supplements,nutraceuticals and medicine is recently considered from the perspective of benefits to human health. Inthis study, the antimicrobial and antioxidant activityof several echinoderm species was investigated.We focused our attention on the two sea urchinsSphaerechinus granularis and Arbacia lixula and onthe sea star Echinaster sepositus. Coelomic fluidand coelomocyte lysate of each species wereutilized for antimicrobial activity assay using theKirby Bauer method (1966). The antioxidant activityof the samples was measured by two in vitroassays: the TEAC (Trolox Equivalent AntioxidantCapacity) assay based on a single electron transfer(SET) reaction, using ABTS [2-2'-azino-bis (3-ethylbenz-thazoline-6-sulfonic acid)] as chromogenand the ORAC (oxygen radical absorbancecapacity) based on a hydrogen transfer mechanism(HAT). Both the antioxidant assays showed a higherantioxidant activity in the coelomocyte lysatecompared to coelomic fluid for all the Echinodermspecies studied. Moreover A. lixula cell lysate hadthe highest antioxidand activity both with TEAC andORAC assay. These antioxidant values arecomparable with those reported in the literature forvarious high antioxidant fruit and spice extracts.Among the investigated species, the coelomocytelysate of A. lixula showed a bacteriostatic activityagainst two emerging pathogenic bacteriaPseudomonas aerugionsa and Staphylococcusaureus and against the yeast Candida famata.These results are noteworthy considering theresistance against antibiotics developed by bacteriaand the need to control human infections. Theantioxidant activity was also of interest since it is thefirst record for the investigated species andrepresents a potential for applicative purposes.

Autore Pugliese

• Gerardi Carmela

Tutti gli autori

• Stabili L.; Acquaviva M.I.; Cavallo R.A.; Gerardi C.; Narracci M.; Pagliara P.

Titolo volume/Rivista

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Anno di pubblicazione

2016

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ISBN

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Numero di citazioni Wos

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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