Abstract

Protein biomarkers are important diagnostic tools for cancer and several other diseases. To be validated in a clinical context, a biomarker should satisfy some requirements including the ability to provide reliable information on a pathological state by measuring its expression levels. In parallel, the development of an approach capable of detecting biomarkers with high sensitivity and specificity would be ideally suited for clinical applications. Here, we performed an immune-based label free assay using Surface Plasmon Resonance (SPR)-based detection of the soluble form of E-cadherin, a cell-cell contact protein that is involved in the maintaining of tissue integrity. With this approach, we obtained a specific and quantitative detection of E-cadherin from a few hundred microliters of serum of breast cancer patients by obtaining a 10-fold enhancement in the detection limit over a traditional colorimetric ELISA.

Autore Pugliese

• Gaballo Antonio , Arima Valentina

Tutti gli autori

• Vergara D.; Bianco M.; Pagano R.; Priore P.; Lunetti P.; Guerra F.; Bettini S.; Carallo S.; Zizzari A.; Pitotti E.; Giotta L.; Capobianco L.; Bucci C.; Valli L.; Maffia M.; Arima V.; Gaballo A.

Titolo volume/Rivista

Nanomedicine

Anno di pubblicazione

2018

ISSN

1549-9634

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile