Abstract

The use of an amphiphilic aryleneethynylene fluorophore as a plasma membrane marker in fixed and livingmammalian cells and liposome model systems is demonstrated. We show here that the optical properties ofthe novel dye are almost independent on pH, in the range 5.0-8.0. Spectroscopic characterization performedon unilamellar liposomes ascertained that the fluorescence intensity of the aryleneethynylene fluorophoregreatly increases after incorporation in lipidic membranes. Experiments performed on different mammaliancells demonstrated that the novel membrane marker exhibits fast staining and a good photostability thatmake it a suitable tool for live cell imaging. Importantly, the aryleneethynylene fluorophore was alsoshown to be a fast and reliable blue membrane marker in classical multicolor immunofluorescence experiments.This study adds new important findings to the recent exploitation of the wide class ofaryleneethynylene molecules as luminescent markers for biological investigations.

Autore Pugliese

• Cardone Antonio

Tutti gli autori

• Cardone A.; Lopez F.; Affortunato F.; Busco G.; Hofer A.M.;Mallamaci R.; Martinelli C.; Colella M.; Farinola G.M.

Titolo volume/Rivista

Biochimica et biophysica acta. Biomembranes

Anno di pubblicazione

2012

ISSN

0005-2736

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile