Abstract

Adiponectin, an adipocyte-derived insulin-sensitizing and anti-inflammatory hormone, is suppressed in obesity through mechanisms involving chronic inflammation and oxidative stress. Olive oil consumption is associated with beneficial cardiometabolic actions, with possible contributions from the antioxidant phenol hydroxytyrosol (HT) and the monounsaturated fatty acid oleic acid (OA, 18:1n-9 cis), both possessing anti-inflammatory and vasculo-protective properties. We determined the effects of HT and OA, alone and in combination, on adiponectin expression in human and murine adipocytes under pro-inflammatory conditions induced by the cytokine tumor necrosis factor(TNF)-?. We used human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes and murine 3T3-L1 adipocytes as cell model systems, and pretreated them with 1-100 ?mol/L OA, 0.1-20 ?mol/L HT or OA plus HT combination before stimulation with 10 ng/mL TNF-?. OA or HT significantly (P<0.05) prevented TNF-?-induced suppression of total adiponectin secretion (by 42% compared with TNF-? alone) as well as mRNA levels (by 30% compared with TNF-? alone). HT and OA also prevented-by 35%-TNF-?-induced downregulation of peroxisome proliferator-activated receptor PPAR?. Co-treatment with HT and OA restored adiponectin and PPAR? expression in an additive manner compared with single treatments. Exploring the activation of JNK, which is crucial for both adiponectin and PPAR? suppression by TNF-?, we found that HT and OA additively attenuated TNF-?-stimulated JNK phosphorylation (up to 55% inhibition). In conclusion, the virgin olive oil components OA and HT, at nutritionally relevant concentrations, have additive effects in preventing adiponectin downregulation in inflamed adipocytes through an attenuation of JNK-mediated PPAR? suppression.

Autore Pugliese

• Scoditti Egeria , Carluccio Maria Annunziata , Massaro Marika

Tutti gli autori

• Scoditti E.; Massaro M.; Carluccio M.A.; Pellegrino M.; Wabitsch M.; Calabriso N.; Storelli C.; De Caterina R.

Titolo volume/Rivista

PloS one

Anno di pubblicazione

2015

ISSN

1932-6203

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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