Abstract

By integrating next generation sequencing (NGS), bioinformatics, electron microscopy and conventional molecular biology tools, a new virus infecting kiwifruit vines has been identified and characterized. Being associated to double membrane-bound bodies in the infected tissues and having a genome composed of RNA segments, each one containing a single open reading frame in the negative polarity, this virus shows the typical features of members of the genus Emaravirus. Five genomic RNA segments were identified. Additional molecular signatures in the viral RNAs and in the proteins they encode, together with data from phylogenetic analyses, support the proposal of creating a new species in the genus Emaravirus for classifying the novel virus, which is tentatively named Actinidia chlorotic ringspot-associated virus (AcCRaV). Bioassays showed that AcCRaV is mechanically transmissible to Nicotiana benthamiana plants that, in turn, may develop chlorotic spots and ringspots. Field survey disclosed the presence of AcCRaV in four different species of kiwifruit vines in five different provinces of central and western China, and supported the association of the novel virus with symptoms of leaf chlorotic ringspots in actinidia. Data on the molecular features of small RNAs of 21-24 nt, derived from AcCRaV RNAs targeted by host RNA silencing mechanisms, are also reported and possible molecular pathways involved in their biogenesis are discussed.

Autore Pugliese

• Navarro Ramirez Beatriz , Di Serio Francesco

Tutti gli autori

• Zheng Y.; Navarro B.; Wang G.; Wang Y.; Yang Z.; Xu W.; Zhu C.; Wang L.; Di Serio F.; Hong N.

Titolo volume/Rivista

Molecular plant pathology

Anno di pubblicazione

2017

ISSN

1464-6722

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile