A selective medium for isolation and accurate enumeration of Lactobacillus casei-group lactobacilli in probiotic milks and dairy products

Abstract

The correct identification and quantitative detection of Lactobacillus casei-group members continues tobe a key issue due to the extensive use of members of this group as (probiotic) adjuncts, corrective and/orreinforcement cultures. Numerous selective/differential media use selective agents to which injured orstressed cells are sensitive, resulting in an underestimation of the actual number of target bacteria. Wedeveloped a selective medium and applied it to detect members of the Lb. casei-group reliably, regardlessof their physiological condition, quantitatively detect and isolate strains of this group from probiotic milkand cheeses, and monitor the probiotic Lb. paracasei CRL 431 strain in Caciotta cheeses made either fromgoats' or cows' milk inoculated with this probiotic strain and a multiple strain culture. This approachcould be useful for control bodies, agro-food and pharmaceutical industries to isolate Lb. casei-groupstrains, develop probiotic food/supplements and demonstrate their compliance with the specieslabelling.


Autore Pugliese

Tutti gli autori

  • Di Lena M.; Quero G.M.; Santovito E.; Verran J.; De Angelis M.; Fusco V.

Titolo volume/Rivista

International dairy journal


Anno di pubblicazione

2015

ISSN

0958-6946

ISBN

Non Disponibile


Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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