A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex

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Abstract

DNA-based phylogenetic analyses have resolved the fungal genus Fusarium into multiple species complexes. TheF. incarnatum-equiseti species complex (FIESC) includes fusaria associated with several diseases of agriculturallyimportant crops, including cereals. Although members of FIESC are considered to be only moderately aggressive,they are able to produce a diversity of mycotoxins, including trichothecenes, which can accumulate to harmfullevels in cereals. High levels of cryptic speciation have been detected within the FIESC. As a result, it is often necessaryto use approaches other than morphological characterization to distinguish species. In the current study,we used a polyphasic approach to characterize a collection of 69 FIESC isolates recovered from cereals in Europe,Turkey, and North America. In a species phylogeny inferred from nucleotide sequences from four housekeepinggenes, 65 of the isolateswere resolvedwithin the Equiseti clade of the FIESC, and four isolateswere resolvedwithinthe Incarnatum clade. Seven isolates were resolved as a genealogically exclusive lineage, designated here asFIESC 31. Phylogenies based on nucleotide sequences of trichothecene biosynthetic genes and MALDI-TOF MS(Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) were largely concordant withphylogeny inferred from the housekeeping gene. Finally, Liquid Chromatography (Time-Of-Flight) Mass Spectrometry[LC-(TOF-)MS(/MS)] revealed variability in mycotoxin production profiles among the different phylogeneticspecies investigated in this study.

Autore Pugliese

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  • A. Villani ; A. Moretti ; S. De Saeger ; Z. Han ; J.D. Di Mavungu ;C.M.G. Soares ; R.H. Proctor ; A. Venâncio ; N. Lima ; G. Stea ; C. Paciolla ;A.F. Logrieco ; A. Susca

Titolo volume/Rivista

International journal of food microbiology

Anno di pubblicazione

2016

ISSN

0168-1605

ISBN

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