Abstract

A cDNA of 312 bp, similar to polygalacturonase-inhibiting proteins(PGIPs), was isolated by cDNA-amplified fragment lengthpolymorphism (cDNA-AFLP) from pea roots infected with thecyst nematode Heterodera goettingiana. The deduced aminoacid sequence obtained from the complete Pspgip1 codingsequence was very similar to PGIPs described from several otherplant species, and was identical in both MG103738 and Progress9 genotypes, resistant and susceptible to H. goettingiana, respectively.Reverse transcription-polymerase chain reaction (RT-PCR)expression analysis revealed the differential regulation of thePspgip1 gene in the two genotypes in response to wounding andnematode challenge. Mechanical wounding induced Pspgip1expression in MG103738 within 8 h, but this response wasdelayed in Progress 9. In contrast, the response to nematodeinfection was more complex. The transcription of Pspgip1 wastriggered rapidly in both genotypes, but the expression levelreturned to levels observed in uninfected plants more quickly insusceptible than in resistant roots. In addition, in situ hybridizationshowed that Pspgip1 was expressed in the cortical cellsdamaged as a result of nematode invasion in both genotypes.However, it was specifically localized in the cells bordering thenematode-induced syncytia in resistant roots. This suggests arole for this gene in counteracting nematode establishmentinside the root.

Autore Pugliese

• Melillo Maria Teresa , Leonetti Paola , Veronico Pasqua

Tutti gli autori

• Veronico P.; Melillo M.T.; Saponaro C.; Leonetti P.; Picardi E.; Jones J.T.

Titolo volume/Rivista

Molecular plant pathology

Anno di pubblicazione

2010

ISSN

1464-6722

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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