A novel nepovirus from Aeonium sp

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Abstract

A virus was isolated from potted plants of an unidentified species of Aeonium (Crassulaceae), a succulent ornamental very common in Southern Italy, showing faint chlorotic spots and rings on both leaf surfaces. The virus was successfully transmitted by sap inoculation to a limited range of hosts, and propagated in Nicotiana benthamiana which was used for ultrastructural observations and virus purification. Virus particles are isometric, ca. 30 nm in diameter, have a single type of coat protein (CP) subunits 54 kDa in size, that encapsidate single-stranded positive-sense RNA species of 7,549 (RNA1) and 4,010 (RNA2) nucleotides. A third RNA molecule 3,472 nts in size entirely derived from RNA2 was also detected in infected Aeonium plants. The structural organization of both genomic RNAs and the cytopathological features were comparable to those of nepoviruses. In addition, amino acid sequence comparisons of CP and the Pro-Pol region (a sequence containing parts of the proteinase and polymerase) with those of other nepoviruses showed that the Aeonium virus belongs to the subgroup A of the genus Nepovirus and is phylogenetically close to Tobacco ringspot virus (TRSV). Comparison of each single domain of TRSV and the Aeonium virus polyproteins disclosed a relatively low percentage of identity throughout. Moreover, the Aeonium virus showed to be serologically distinct from TRSV. Based on the species demarcation criteria for the family Secoviridae, the virus under study appears to be a novel member of the genus Nepovirus for which the name of Aeonium ringspot virus (AeRSV) is proposed.

Autore Pugliese

Tutti gli autori

  • Sorrentino R.; De Stradis A.; Russo M.; Alioto D.; Rubino L.

Titolo volume/Rivista

Journal of plant pathology

Anno di pubblicazione

2013

ISSN

1125-4653

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Settori ERC

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Codici ASJC

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