Abstract

In addition to the full-length transcript ARF8.1, a splice variant (ARF8.2) of the auxin response factor gene ARF8 has beenreported. Here, we identified an intron-retaining variant of ARF8.2, ARF8.4, whose translated product is imported into thenucleus and has tissue-specific localization in Arabidopsis thaliana. By inducibly expressing each variant in arf8-7 flowers, weshow that ARF8.4 fully complements the short-stamen phenotype of the mutant and restores the expression of AUX/IAA19,encoding a key regulator of stamen elongation. By contrast, the expression of ARF8.2 and ARF8.1 had minor or no effects onarf8-7 stamen elongation and AUX/IAA19 expression. Coexpression of ARF8.2 and ARF8.4 in both the wild type and arf8-7caused premature anther dehiscence: We show that ARF8.2 is responsible for increased expression of the jasmonic acidbiosynthetic gene DAD1 and that ARF8.4 is responsible for premature endothecium lignification due to precocious expressionof transcription factor gene MYB26. Finally, we show that ARF8.4 binds to specific auxin-related sequences in both the AUX/IAA19 and MYB26 promoters and activates their transcription more efficiently than ARF8.2. Our data suggest that ARF8.4 isa tissue-specific functional splice variant that controls filament elongation and endothecium lignification by directlyregulating key genes involved in these processes.

Autore Pugliese

• De Paolis Angelo

Tutti gli autori

• R. Ghelli; P. Brunetti; N. Napoli; A. De Paolis; V. Cecchetti; T. Tsuge; G. Serino; M. Matsui; G. Rinaldi; G.A. Palumbo; F. Barozzi; P. Costantino ; M. Cardarelli

Titolo volume/Rivista

The Plant cell

Anno di pubblicazione

2018

ISSN

1532-298X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile