Abstract

Perfectly integrated in the scenario of modern research, which requires advancedtechnologies to be applied in a wide range of research fields, the Institute forBiomedical Technologies in Bari has equipped its Labs with both the 454 GenomeSequencer FLX Titanium by Roche and with a powerful bioinformatics platform(hardware and software facilities) for managing and analysing NGS data.To cope with the fast rate at which NGS technologies are evolving, thedevelopment and setting up of new experimental protocols and of bioinformaticsanalysis pipelines is a challenge that researchers have to face daily.Major obstacles in NGS "omics" research are - at experimental level - finding thebest way to extract DNA or RNAs from samples and obtaining good libraries forsequencing, and - at bioinformatics level - data storage, transfer, and analysis. Theclassical statistical methods and computational algorithms are inadequate foranalysing the large amount of sequence data produced by new NGS technologies.Novel analytical strategies are urgently needed for exploring new features ofsequencing data, integrating various genomic and epigenomic data, unravelling thestructure, organisation, and function of the human genome, understandingfundamental principles of genomic biology, and discovering genetic and nongeneticbases of diseases.The Genomics Research team of ITB in Bari, is gaining great acquaintance withhigh throughput sequencing procedure and it has developed a new protocol for thepreparation and amplification of representative cDNA libraries to be sequenced byNGS platforms. This protocol is patent pending in Europe.The Bioinformatics Group is focused on the development of bioinformatics tools foranalysing data obtained by different NGS platforms for diverse projects spanningfrom molecular studies in cancer research to biodiversity studies. In this respect,the ITB-BA, in collaboration with other CNR and Academic Institutions, is presentlyinvolved in several projects for studying the molecular biodiversity in metagenomicsand metatrascriptomics within Biomedical, Food and Environmental fields.In particular we are:- studying the transcriptome of normal and pathological samples with the aim ofidentifying genes, new mRNA isoforms, microRNA and genome wide mapping oftranscription factors involved in the etiopathogenesis of human diseases;- analysing the exome and the transcriptome profile in short children with particularattention to the involvement of the p53 oncosuppressor gene family members (p53,p63, p73) in the regulation of the genes involved in growth;- investigating the possibility that only particular viral genotypes of Epstein Barrvirus (EBV), that ubiquitously infects humans, can be associated with theetiopathogenesis of multiple sclerosis;- investigating the taxonomical complexity of microbial communities living in foodindustry "habitats", particularly in winemaking chain, shedding li

Autore Pugliese

• D'elia Domenica

Tutti gli autori

• D. D'Elia; M. Mangiulli; M. Santamaria; A.M. Paluscio; C. Manzari; M.F. Caratozzolo; F. Marzano;M. Marzano; E. Perlino; P. Fuzio; A. Valletti; A.M. D'Erchia; A. Consiglio; A. Gisel; S. Vicario; B. Balech;F. Licciulli; G. Grillo; S. Liuni; E. Picardi; G. Pesole;E. Sbisà; A. Tullo

Titolo volume/Rivista

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Anno di pubblicazione

2011

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ISBN

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Numero di citazioni Wos

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Settori ERC

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