Abstract

Abstract Adipose tissue is a complex biological matrix that necessitates several pre-analytical preparation steps to separate drugs and metabolites from the lipophilic matrix. A novel, sensitive, and specific gas chromatographic-mass spectrometric (GC-MS) method for the determination of cocaine (metabolites), methadone, and morphine in postmortem adipose tissue was developed, optimized, and validated. The method involves the aqueous acid extraction of analytes, alkalinization of the extract, solid-phase extraction with chloroform, and derivatization with BSTFA before GC-MS analysis. Deuterated compounds were used as internal standards for determination and quantification of analytes. Limits of detection were 0.005 µg/g for cocaine and cocaethylene, 0.02 µg/g for benzoylecgonine, 0.01 µg/g for ecgoninemethylester, 0.005 µg/g for methadone, and 0.01 µg/g for morphine. Linearity ranged from 0.1 to 1.000 µg/g for all analytes. Intra- and interday accuracy ranged from 70.6 to 105%, and intra- and interday precisions were less than 8.2% and 8.6%, respectively, for all analytes. The method showed a good recovery

Autore Pugliese

• Aventaggiato Lucia

Tutti gli autori

• AVENTAGGIATO L.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2010

ISSN

0146-4760

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

8

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile