Abstract

A new optical biosensor for trehalose determination has been realized immobilizing three glycoenzymeson a transparent support. Trehalase, glucose oxidase and horseradish peroxidase have been alternatedwith layers of Concanavalin A by a “layer-by-layer” (LbL) deposition. The driving force of this assembly isthe biospecific complexation between Concanavalin A and sugar residues in the glycoenzymes. As con-firmed by UV–vis spectroscopy, the LbL deposition allowed a high ordinate architecture with high loadingof enzymes. After the assembly, the functionality of immobilized enzymes was spectrophotometricallyproven, demonstrating also that they can act in series catalyzing cascade reactions.The prepared biosensor was used to optically detect trehalose, giving a LOD of 10 M and a linearresponse up to 4 mM, and it showed also good time stability. The trehalose content in a real sample(eyewash) was successfully determined by the biosensor.

Autore Pugliese

• Palazzo Gerardo , Colafemmina Giuseppe

Tutti gli autori

• PALAZZO G.;COLAFEMMINA G.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2014

ISSN

0925-4005

ISBN

Non Disponibile

Numero di citazioni Wos

11

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

11

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile