Abstract

Metal nanomaterials have emerging role in surface-assisted laser desorption ionisation–mass spectrometry (SALDI-MS) providing a useful tool to overcome some limitations intrinsically related to the use of conventional organic matrices in matrix-assisted LDI-MS. In this contribution, the possibility to use a stainless steel-supported gold nanoparticle (AuNP) film as a versatile platform for SALDI-MS was assessed. Sacrificial anode electrosynthetic route was chosen in order to obtain morphologically controlled core–shell AuNPs; the colloidal AuNPs were, thereafter, drop cast onto a stainless steel sample plate and the resulting AuNP film was thermally annealed in order to improve its effectiveness as LDI-MS promoter. Spectroscopic characterization of the nanostructured film by X-ray photoelectron spectroscopy was crucial for understanding how annealing induced changes in the surface chemistry and influenced the performance of AuNPs as desorption/ionisation promoter. In particular, it was demonstrated that the post-deposition treatments were essential to enhance the AuNP core/analyte interaction, thus resulting in SALDI-MS spectra of significantly improved quality. The AuNP films were applied to the detection of three different classes of low molecular weight (LMW) analytes, i.e. amino acids, peptides and LMW polymers, in order to demonstrate the versatility of this nanostructured material.

Autore Pugliese

• Palmisano Francesco , Cioffi Nicola , Ditaranto Nicoletta

Tutti gli autori

• DI FRANCO C.;PALMISANO F.;CIOFFI N.;DITARANTO N.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2012

ISSN

1618-2642

ISBN

Non Disponibile

Numero di citazioni Wos

16

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

17

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile