Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles

Abstract

Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0-2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.


Tutti gli autori

  • CAVALLI A.;MARSILIO F.;DIAKOUDI G.;LANAVE G.;LANAVE G.;MARTELLA V.

Titolo volume/Rivista

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Anno di pubblicazione

2018

ISSN

0168-1702

ISBN

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Settori ERC

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Codici ASJC

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