Abstract

Background: Legionella is anintracellularmicroorganismlivinginnaturalandartificial aquaticen- vironments. Althoughitstransmissiontohumansislinkedtotheinhalationofcontaminatedaerosols, there isnovalidatedairsamplingmethodforthecontrolandpreventionofthedisease.Theaimofthe present studywastoprovidemoreinformationonthedistributionof Legionella spp. inindooren- vironments andtodeterminewhetherthesame Legionella strains areisolatedfromairandwater samples. Methods: TenhealthcarefacilitieslocatedinsevenregionsofItalywereenrolled.Theserologicaltyping of Legionella spp. fromwatersamplesandthesurroundingairbyactiveandpassivesamplingwasas- sessed usingpolyvalentandmonovalentantisera.Subsequently,thestrainsidentified as Legionella pneumophila (Lpn) underwentmoleculartypingbysequence-basedtyping(SBT)usingsevengenes(flaA, pilE, asd, mip, mompS, proA, and neuA). Theallelicprofile numberwasassignedusingtheEuropean WorkingGroupfor Legionella Infections–SBT database. Results: Lpn serogroup6wasthemostprevalentserogroup;itwasfoundsimultaneouslyintheairand watersamplesofthreedifferenthealthcarefacilities.Intheremainingsevenhospitals, Lpn serogroups1, 6, 7,9,and12wereisolatedexclusivelyfromwatersamples.Themolecularinvestigationshowedthat Lpn strains inthewaterandairsamplesofeachpositivehealthcarefacilityhadthesameallelicprofile. Strains,identified assequencetypes(STs)728andST1638þST 1324,wereisolatedintworespective healthcare facilities,andanewstrain,identified asST1989,wasobtainedinonehealthcarefacility

Autore Pugliese

• Montagna Maria Teresa , De Giglio Osvalda

Tutti gli autori

• NAPOLI C.;MONTAGNA M.T.;DE GIGLIO O.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2016

ISSN

0013-9351

ISBN

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Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

18

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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