Abstract

ADARs are key proteins for hematopoietic stem cell self-renewal and for survival of differentiating progenitor cells. However, their specific role in myeloid cell maturation has been poorly investigated. Here, we show that ADAR1 is present at basal level in the primary myeloid leukemia cells obtained from patients at diagnosis as well as in myeloid U-937 and THP1 cell lines and its expression correlates with the editing levels. Upon phorbol-myristate acetate (PMA) or VitaminD3/GM-CSF-driven differentiation, both ADAR1 and ADAR2 enzymes are up-regulated, with a concomitant global increase of A-to-I RNA editing. ADAR1-silencing caused an editing decrease at specific ADAR1 target genes, without, however, interfering with cell differentiation or with ADAR2 activity. Remarkably, ADAR2 is absent in the undifferentiated cell stage, due to its elimination through the ubiquitin-proteasome pathway, being strongly up-regulated at the end of the differentiation process. Of note, peripheral blood monocytes display editing events at the selected targets similar to those found in differentiated cell lines. Taken together, the data indicate that ADAR enzymes play important and distinct roles.Leukemia accepted article preview online, 09 May 2017. doi:10.1038/leu.2017.134.

Autore Pugliese

• D'erchia Anna Maria , Picardi Ernesto , Pesole Graziano

Tutti gli autori

• D'ERCHIA A.M.;PICARDI E.;PESOLE G.;SORRENTINO M.R.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2017

ISSN

0887-6924

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

7

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

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Codici ASJC

Non Disponibile