Abstract

A Functional Bio-Interlayer Organic Field-Effect Transistor (FBI-OFET) sensor, embedding a streptavidin protein capturing layer, capable to perform label-free specific electronic detection of biotin at 3 part-per-trillion (mass fraction) or 15 pM, is here proposed. The response shows a logarithmic dependence on the analyte concentration along with a dynamic range spanning over five orders of magnitude. The optimization of the FBI analytical performances is achieved by depositing the capturing layer through a controllable Layer-by-Layer (LbL) assembly, while an easy processable spin-coating deposition is proposed for potential low-cost production of equally highly performing sensors. A Langmuirian adsorption based model allows to rationalize the analyte binding to the capturing layer whose analytical performances are discussed accordingly. The FBI-OFET device is shown to operate also with an antibody interlayer as well as with an ad hoc designed micro-fluidic system. These occurrences, along with the proven extremely high sensitivity and selectivity, open to FBI-OFETs appraisal as a suitable platform for disposable electronic strip-tests for assays in biological fluids requiring very low detection limits.

Autore Pugliese

• Palazzo Gerardo , Sabbatini Luigia , Torsi Luisa , Cioffi Nicola

Tutti gli autori

• PALAZZO G.;SABBATINI L.;TORSI L.;MAGLIULO M.;CIOFFI N.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2013

ISSN

0003-2700

ISBN

Non Disponibile

Numero di citazioni Wos

34

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

36

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile