Abstract

Label-free biosensors are of considerable interest for various clinical and biological applications. In these systems, achieving an optimized receptor immobilization strategy critically influence the sensing performance in terms of specificity, sensitivity, response kinetics and detection limits. However, monitoring the receptor spatial organization and the interfaces composition on a nanometer or sub-nanometer scale is a very hard challenge. In the present contribution Parallel Angle Resolved X-ray Photoelectron Spectroscopy (PAR-XPS) was proposed as useful tool to address the challenge of probing the near-surface region of bio-active sensors surface (1). A model receptor was chosen and a well-established functionalization procedure (2) was systematically characterized by PAR-XPS. Commercially available Thermo Avantage-ARProcess software was used to generate non-destructive concentration depth profiles of protein functionalized silicon oxide substrates. At each step of the functionalization procedure, the surface composition, the over layer thickness, the in-depth organization and the in-plane homogeneity were evaluated. Compared to multi-techniques characterization approaches previously proposed in the literature, the present analytical approach boasted the peculiar advantage of providing, simultaneously, morphological and compositional information from the same data set. The critical discussion of the generated profiles highlighted the relevance of the information provided by PAR-XPS technique.

Autore Pugliese

• Sabbatini Luigia , Cioffi Nicola , Ditaranto Nicoletta

Tutti gli autori

• SABBATINI L.;CIOFFI N.;DITARANTO N.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2012

ISSN

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ISBN

978-88-907670-8-1

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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