Abstract

The purpose of this work is to explore the mass fragment characterization of commonly used drugs through a novel approach which involves isotope-selective tandem mass spectrometry (MS/MS). Collision induced dissociation (CID) was performed with a low-resolution linear-ion-trap mass spectrometer in positive electrospray ionization (ESI). Three pharmacologically active ingredients, i.e., omeprazole, meloxicam and brinzolamide, selected as model compounds in their own formulation, were investigated as a sodiated adduct [C17H19N3O3S+Na]+ (omeprazole) and as protonated adducts, [C14H13N3O4S2+H]+ and [C12H21N3O5S3+H]+, meloxicam and brinzolamide, respectively. Selecting a narrow window of 0.5 m/z units, precursor ion fragmentation by CID-MS/MS of isotopologues A+0, A+1 and A+2 was found very useful to confirm the chemical formula of product ions, thus aiding the establishment of characteristic fragmentation pathways of all three examined compounds. The correctness of putative molecular formula of product ions was easily demonstrated by exploiting the isotope peak abundance ratios (i.e., IF+0/IF+1 and IF+0/IF+2) as simple constraints in low resolution MS instrumentations.

Autore Pugliese

• Cataldi Tommaso

Tutti gli autori

• CATALDI T.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2014

ISSN

1096-9888

ISBN

Non Disponibile

Numero di citazioni Wos

2

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

2

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile