Abstract

A method of direct lipid analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry (MS) in intact membranes, without prior extraction/separation steps, is described. Here, we demonstrate the efficacy of a strong base, 1,8‐bis(dimethylamino)naphthalene(DMAN; proton sponge), as a novel matrix for MALDI‐time‐of‐flight (TOF) MS analysis of whole cell bacteria. Initially, individual acidic low‐molecular‐weight analytes such as standard free fatty acids and phospholipids were analyzed using DMAN asmatrix. Clear negative‐modeMALDI‐TOFMS spectra of all analytes show only deprotonated analyte signals at a low picomole limit of detection with the complete absence of matrix‐related signals. These results indicate that DMAN represents a suitable matrix for MALDI‐TOF MS analysis of mixtures of complex lipids as the intact membranes of microorganisms. DMAN was successfully applied to the analysis of Lactobacillus sanfranciscensis and L. plantarum microorganisms. Different components were sensitively detected in a single spot, including 16:0, 18:2, 18:3, and 21:0 free acids, glycolipids, phosphatidylglycerols (PGs) and cardiolipins. This method might be of general application, offering the advantage of quickly gaining information about lipid components of other Gram‐positive bacterial membranes.

Autore Pugliese

• Calvano Cosima Damiana , Palmisano Francesco

Tutti gli autori

• CALVANO C.D.;PALMISANO F.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2011

ISSN

0951-4198

ISBN

Non Disponibile

Numero di citazioni Wos

30

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

32

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile