Abstract

Biosystems integration into an organic field-effect transistor (OFET) structure is achieved by spin coating phospholipid or protein layers between the gate dielectric and the organic semiconductor. An architecture directly interfacing supported biological layers to the OFET channel is proposed and, strikingly, both the electronic properties and the biointerlayer functionality are fully retained. The platform bench tests involved OFETs integrating phospholipids and bacteriorhodopsin exposed to 1–5% anesthetic doses that reveal drug-induced changes in the lipid membrane. This result challenges the current anesthetic action model relying on the so far provided evidence that doses much higher than clinically relevant ones (2.4%) do not alter lipid bilayers’ structure significantly. Furthermore, a streptavidin embedding OFET shows label-free biotin electronic detection at 10 parts-per-trillion concentration level, reaching state-of-the-art fluorescent assay performances. These examples show how the proposed bioelectronic platform, besides resulting in extremely performing biosensors, can open insights into biologically relevant phenomena involving membrane weak interfacial modifications.

Autore Pugliese

• Scamarcio Gaetano , Palazzo Gerardo , Sabbatini Luigia , Torsi Luisa , Cioffi Nicola

Tutti gli autori

• SCAMARCIO G.;PALAZZO G.;SABBATINI L.;TORSI L.;MAGLIULO M.;CIOFFI N.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2012

ISSN

1091-6490

ISBN

Non Disponibile

Numero di citazioni Wos

72

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

77

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile