Abstract

Aims: Sepsis is the leading cause of death in intensive care units usually related to the number and the severity of organ failure, but the mechanisms remain to be fully established. Findings of microvascular flow abnormalities, decreased oxygen consumption and elevated tissue oxygen tensions suggest that problems may lay in cellular oxygen utilization rather than in oxygen delivery per se. Several serum factors, released during sepsis syndrome, might be involved in induction of cytopathic hypoxia and increase of cellular oxidative stress. Main methods: Human fibroblast cultures were incubated 12 hours with 10% v/v severe septic patients sera and measurements were carried out on cellular oxygen consumption, mitochondrial respiratory enzymes activity, H2O2 generation and serum levels of cytokines/chemokines by multiplex assay. Key findings: In fibroblast cultures a significant depression of cellular respiration and activity of mitochondrial complexes and increased H2O2 production was observed and, IL-1after incubation with septic sera showing increased levels of TNF IL-6. Significance: During sepsis syndrome some increased cytokines might target specific mitochondrial enzymes inducing an impairment of cellular energy metabolism leading to multiple organ failure

Autore Pugliese

• Papa Francesco , Santacroce Luigi , Brienza Nicola , Scacco Salvatore

Tutti gli autori

• PAPA F.;SANTACROCE L.;BRIENZA N.;SCACCO S.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2012

ISSN

0024-3205

ISBN

Non Disponibile

Numero di citazioni Wos

11

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

14

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile