Genetic analysis and molecular characterization of laboratory and field mutants of Botryotinia fuckeliana (Botrytis cinerea) resistant to QoI fungicides

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Abstract

BACKGROUND: QoI fungicides, inhibitors of mitochondrial respiration, are considered to be at high risk of resistance development. In several phytopathogenic fungi, resistance is causedby mutations (most frequentlyG143A)inthemitochondrial cytochrome b (cytb) gene. The genetic and molecular basis of QoI resistance were investigated in laboratory and field mutants of Botryotinia fuckeliana (de Bary)Whetz. exhibiting in vitro reduced sensitivity to trifloxystrobin. RESULTS: B. fuckeliana mutants highly resistant to trifloxystrobin were obtained in the laboratory by spontaneous mutations in wild-type strains, or from naturally infected plants on a medium amended with 1–3 mg L−1 trifloxystrobin and 2mM salicylhydroxamic acid, an inhibitor of alternative oxidase. No point mutations were detected, either in the complete nucleotide sequences of the cytb gene or in those of the aox and Rieske protein genes of laboratory mutants, whereas all field mutants carried the G143A mutation in the mitochondrial cytb gene. QoI resistance was always maternally inherited in ascospore progeny of sexual crosses of field mutants with sensitive reference strains. CONCLUSIONS: The G143A mutation in cytb gene is confirmed to be responsible for field resistance to QoIs in B. fuckeliana. Maternal inheritance of resistance to QoIs in progeny of sexual crosses confirmed that it is caused by extranuclear genetic determinants. Inlaboratory mutants theheteroplasmic state of mutated mitochondria could likelyhampertheG143Adetection, otherwise other gene(s) underlying different mechanisms of resistance could be involved.

Autore Pugliese

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  • FARETRA F.;DE MICCOLIS ANGELINI R.M.;POLLASTRO S.

Titolo volume/Rivista

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Anno di pubblicazione

2012

ISSN

1526-498X

ISBN

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Numero di citazioni Wos

14

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Numero di citazioni Scopus

17

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Settori ERC

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Codici ASJC

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