Elements in support of the 'non-identity' of the PGRMC1 protein with the σ<inf>2</inf> receptor

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Abstract

σ2 Receptorsubtypeisoverexpressedinavarietyofhumantumors,with σ2 agonists showing antiproliferativeeffectstowardstumorcellsthroughmultiplepathwaysthatdependbothonthetumor cell typeandonthemoleculetype.Therefore, σ2 receptorisanintriguingtargetfortumordiagnosisand treatment despitethefactthatthatithasnotyetbeencloned.Oneofthelastattemptstocharacterize σ2 receptorsledtoidentifyitastheprogesteronereceptormembranecomponent1(PGRMC1).Although still controversial,suchidentityappearstohavebeenaccepted.Wetheaimofcontributingtosolvethis controversy,inthisworkwestablysilencedoroverexpressedPGRMC1proteininhumanMCF7 adenocarcinoma cells.WesternblottinganalyseswereperformedtoquantifythepresenceofPGRMC1 proteinoneachofthethreeMCF7celllinesvariants,whilescatchardanalyseswithradioligandwere performed inordertodeterminetheexpressionofthe σ2 receptors.Inordertocorrelatethe antiproliferativeeffectof σ2 receptoragonistwithPGRMC1density,some σ2 ligands wereadministered to eachofthethreeMCF7cellsvariants.TheresultssuggestedthatPGRMC1and σ2 receptorsaretwo different molecularentities.

Autore Pugliese

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  • MENGA A.;ABATE C.;BERARDI F.;INFANTINO V.

Titolo volume/Rivista

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Anno di pubblicazione

2015

ISSN

0014-2999

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

37

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Settori ERC

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Codici ASJC

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