Abstract

tThe aim of this work is to study the nature of reactive oxygen species, ROS, arisen from Chitosan/2-HP--Cyclodextrin/Chlorophyll a (CH/CD/Chla) blended biofilm under a photodynamic activity. Suitablemolecules, called primary acceptors, able to react selectively with ROS, in turn generated by the pho-tosensitizer (PS), herein Chla, are used to attempt this purpose. The changes of the absorption and theemission spectra of these acceptors after the irradiation of aqueous solution containing the active biofilmhave provided the specific nature of ROS and thus the main pathway of reaction followed by PS, in ourcondition. The1O2formation was unveiled using Uric Acid (UA) and 9,10-diphenilanthracene (DPA). Onthe other hand, 2,7- dichlorofluorescin and Ferricytochrome c (Cyt-c) were used to detect the formationof hydrogen peroxide and superoxide radical anion, respectively. Results suggest that among the possiblepathways of reaction, namely Type I and Type II, potentially followed by PSs, in our condition the hybridbiofilm CH/CD/Chla follows mainly Type II mechanism with the formation of1O2. However, the latter isinvolved in subsequent pathway of reaction involving Chla inducing, in addition, the formation of O2•−and H2O2.

Autore Pugliese

• Cosma Pinalysa

Tutti gli autori

• SEMERARO P.;COSMA P.;RIZZI V.;RIZZI V.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2016

ISSN

0927-7765

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

7

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile